Biological Tissues

abtc-labeling-vials-430W

Tiny slivers of tissue (sub-samples) are cut from samples in the collection for use in a wide range of genetic research projects.

Australian Biological Tissue Collection

The Australian Biological Tissue Collection (ABTC) was the first frozen tissue collection in any Australian museum and is one of the largest wildlife tissue collections in the world. This important collection encompasses most vertebrate groups as well as a broad representation of major invertebrate groups from Australia and surrounding regions. It has immense research significance for researchers around the world.

The ABTC is the largest tissue bank for wildlife research in the Southern Hemisphere. It contains nearly 125,000 samples of animal and plant samples collected from terrestrial and marine animals.  The ABTC is managed by two staff in the Museum’s Evolutionary Biology Unit.

The samples are carefully maintained by Collection Manager Leanne Wheaton to ensure their quality is suitable for detailed biological research using molecular genetic technologies. This involves determining the genetic profiles of samples, either indirectly from structural elements of cells and tissues (proteins), or directly from the primary genetic material of the cells (DNA and RNA).

Most ABTC tissues are stored in ultra-freezers, where the temperature is maintained at approximately -70°C. At this temperature, animal cell structures remain intact, making frozen tissues suitable for protein, DNA and RNA analysis. A smaller proportion of the ABTC is maintained in concentrated alcohol — these samples are typically used for DNA analysis only.

ABTC freezer.

Accessing frozen tissue samples in one of the ABTC’s freezers maintained at approximately -70°C.  

The South Australian Museum’s ABTC is a resource of global importance because of its size, its focus on native Australian fauna, the time span over which good quality tissue collection has taken place (more than 30 years), and its well-organised storage system. Tissue samples from introduced species and animals from other regions such as New Guinea are also a feature of the collection.

 

History

Collection of ABTC samples began in 1979. Even though DNA analysis was not routine back then, former South Australian Museum scientists Terry Schwaner and Peter Baverstock had the foresight to not only collect animal specimens, but also to freeze tissue samples for genetic analysis. The development of a well-curated collection of high quality frozen tissue samples gives today’s scientists the opportunity to obtain a snapshot of the distributions and genetic makeup of many species — some of which may now be endangered or extinct — from 30–40 years ago.

Tissue collection for the ABTC continues today, under animal ethics guidelines, by virtue of surveys and environmental impact studies by government and private organisations, amateur scientific societies and targeted collecting by scientists with specific research interests.

 

Staff responsible for the ABTC